Role of artemin in non-small cell lung cancer.

ABSTRACT:

Background

In this study, we investigated the role of artemin, a member of the glial cell-derived neurotrophic factor of ligands, in the malignant phenotype of lung cancer.

Methods

Artemin expression was examined in various types of lung cancer and normal lung tissues, as well as in lung cancer cell lines by immunohistochemistry and semi-quantitative PCR. Functional studies were performed using artemin overexpression or knockdown vectors in lung cancer cell lines. Methyl thiazolyl tetrazolium, flow cytometry, wound healing, and transwell assays were conducted to evaluate the contribution of artemin on tumor cell proliferation, migration, and invasion.

Results

Artemin is broadly expressed in lung cancer tissues, and is associated with tumor staging. Overexpression of a

SUBMITTER: Song Z 

PROVIDER: S-EPMC5928368 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature