The Versatility of Framework Regions of Chicken V_H and V_L to Mutations.

ABSTRACT: To identify the interchangeability of V_H and V_L framework region (FR) residues, we artificially introduced random mutations at all residue positions in a chicken monoclonal antibody, which has only one functional V_H and V_λ gene. When we classified the amino acids into 5 groups by their physicochemical properties, all FR residues could be replaced by another group except L23 (C), H36 (W), H86 (D), H104 (G), and H106 (G). Eighty-two (50.9%), 48 (29.8%), 17 (10.6%), and 9 FR residues (5.6%) could be replaced by 4, 3, 2, and 1 group(s), individually, without significant loss of reactivity. We also confirmed a similar level of versatility with 2 different chicken antibodies. This high level of versatility on FR residues has not been predicted because

SUBMITTER: Shin JW 

PROVIDER: S-EPMC5928418 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature