KatG-Mediated Oxidation Leading to Reduced Susceptibility of Bacteria to Kanamycin.

ABSTRACT: Resistance to antibiotics has become a serious problem for society, and there are increasing efforts to understand the reasons for and sources of resistance. Bacterial-encoded enzymes and transport systems, both innate and acquired, are the most frequent culprits for the development of resistance, although in Mycobacterium tuberculosis, the catalase-peroxidase, KatG, has been linked to the activation of the antitubercular drug isoniazid. While investigating a possible link between aminoglycoside antibiotics and the induction of oxidative bursts, we observed that KatG reduces susceptibility to aminoglycosides. Investigation revealed that kanamycin served as an electron donor for the peroxidase reaction, reducing the oxidized ferryl intermediates of KatG to the resting state. Loss of electro

SUBMITTER: Loewen PC 

PROVIDER: S-EPMC5928485 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature