Green monomeric photosensitizing fluorescent protein for photo-inducible protein inactivation and cell ablation.

ABSTRACT:

Background

Photosensitizing fluorescent proteins, which generate reactive oxygen species (ROS) upon light irradiation, are useful for spatiotemporal protein inactivation and cell ablation. They give us clues about protein function, intracellular signaling pathways and intercellular interactions. Since ROS generation of a photosensitizer is specifically controlled by certain excitation wavelengths, utilizing colour variants of photosensitizing protein would allow multi-spatiotemporal control of inactivation. To expand the colour palette of photosensitizing protein, here we developed SuperNova Green from its red predecessor, SuperNova.

Results

SuperNova Green is able to produce ROS spatiotemporally upon blue light irradiation. Based on protein characterization, SuperNova Green

SUBMITTER: Riani YD 

PROVIDER: S-EPMC5928576 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature