Release of MicroRNAs into Body Fluids from Ten Organs of Mice Exposed to Cigarette Smoke.

ABSTRACT: Purpose: MicroRNAs are small non-coding RNAs that regulate gene expression, thereby playing a role in a variety of physiological and pathophysiological states. Exposure to cigarette smoke extensively downregulates microRNA expression in pulmonary cells of mice, rats, and humans. Cellular microRNAs are released into body fluids, but a poor parallelism was previously observed between lung microRNAs and circulating microRNAs. The purpose of the present study was to validate the application of this epigenetic biomarker by using less invasive collection procedures. Experimental design: Using microarray analyses, we measured 1135 microRNAs in 10 organs and 3 body fluids of mice that were either unexposed or exposed to mainstream cigarette smoke for up to 8 weeks. The results obtain

SUBMITTER: Izzotti A 

PROVIDER: S-EPMC5928877 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature