Mouse genetic background influences whether <i>Hras^G12V</i> expression plus <i>Cdkn2a</i> knockdown causes angiosarcoma or undifferentiated pleomorphic sarcoma.

ABSTRACT: Soft tissue sarcomas are rare mesenchymal tumours accounting for 1% of adult malignancies and are fatal in approximately one third of patients. Two of the most aggressive and lethal forms of soft tissue sarcomas are angiosarcomas and undifferentiated pleomorphic sarcomas (UPS). To examine sarcoma-relevant molecular pathways, we employed a lentiviral gene regulatory system to attempt to generate in vivo models that reflect common molecular alterations of human angiosarcoma and UPS. Mice were intraveneously injected with MuLE lentiviruses expressing combinations of shRNA against Cdkn2a, Trp53, Tsc2 and Pten with or without expression of Hras^G12V , PIK3CA^H1047R or Myc. The systemic injection of an ecotropic lentivir

SUBMITTER: Brandt LP 

PROVIDER: S-EPMC5929423 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature