Candidate gene identification of ovulation-inducing genes by RNA sequencing with an in vivo assay in zebrafish.

ABSTRACT: We previously reported the microarray-based selection of three ovulation-related genes in zebrafish. We used a different selection method in this study, RNA sequencing analysis. An additional eight up-regulated candidates were found as specifically up-regulated genes in ovulation-induced samples. Changes in gene expression were confirmed by qPCR analysis. Furthermore, up-regulation prior to ovulation during natural spawning was verified in samples from natural pairing. Gene knock-out zebrafish strains of one of the candidates, the starmaker gene (stm), were established by CRISPR genome editing techniques. Unexpectedly, homozygous mutants were fertile and could spawn eggs. However, a high percentage of unfertilized eggs and abnormal embryos were produced from these homozygous females. The r

SUBMITTER: Klangnurak W 

PROVIDER: S-EPMC5929532 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature