Robustness encoded across essential and accessory replicons of the ecologically versatile bacterium Sinorhizobium meliloti.

ABSTRACT: Bacterial genome evolution is characterized by gains, losses, and rearrangements of functional genetic segments. The extent to which large-scale genomic alterations influence genotype-phenotype relationships has not been investigated in a high-throughput manner. In the symbiotic soil bacterium Sinorhizobium meliloti, the genome is composed of a chromosome and two large extrachromosomal replicons (pSymA and pSymB, which together constitute 45% of the genome). Massively parallel transposon insertion sequencing (Tn-seq) was employed to evaluate the contributions of chromosomal genes to growth fitness in both the presence and absence of these extrachromosomal replicons. Ten percent of chromosomal genes from diverse functional categories are shown to genetically interact with pSymA and pSymB. T

SUBMITTER: diCenzo GC 

PROVIDER: S-EPMC5929573 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature