Effects of nandrolone decanoate on expression of steroidogenic enzymes in the rat testis.

ABSTRACT:

Objective

Nandrolone decanoate (ND) is an anabolic-androgenic steroid frequently used for clinical treatment. However, the inappropriate use of ND results in the reduction of serum testosterone level and sperm production. The suppressive effect of ND on testosterone production has not been investigated in detail. The present study was designed to examine the effect of ND on the expression of steroidogenic enzymes in the rat testis.

Methods

Male Sprague Dawley rats at 50 days of age were subcutaneously administrated with either 2 or 10 mg of ND/kg body weight/week for 2 or 12 weeks. The changes of transcript and protein levels of steroidogenic enzymes in the testis were determined by real-time polymerase chain reaction and western blotting analyses, respectively. Moreover, im

SUBMITTER: Min T 

PROVIDER: S-EPMC5930276 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature