The Lysine 299 Residue Endows the Multisubunit Mrp1 Antiporter with Dominant Roles in Na⁺ Resistance and pH Homeostasis in Corynebacterium glutamicum.

ABSTRACT: Corynebacterium glutamicum is generally regarded as a moderately salt- and alkali-tolerant industrial organism. However, relatively little is known about the molecular mechanisms underlying these specific adaptations. Here, we found that the Mrp1 antiporter played crucial roles in conferring both environmental Na⁺ resistance and alkali tolerance whereas the Mrp2 antiporter was necessary in coping with high-KCl stress at alkaline pH. Furthermore, the Δmrp1 Δmrp2 double mutant showed the most-severe growth retardation and failed to grow under high-salt or alkaline conditions. Consistent with growth properties, the Na⁺/H⁺ antiporters of C. glutamicum were differentially expressed in response to specific salt or alkaline stress, and

SUBMITTER: Xu N 

PROVIDER: S-EPMC5930375 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature