Establishment of Dimethyl Labeling-based Quantitative Acetylproteomics in Arabidopsis.

ABSTRACT: Protein acetylation, one of many types of post-translational modifications (PTMs), is involved in a variety of biological and cellular processes. In the present study, we applied both CsCl density gradient (CDG) centrifugation-based protein fractionation and a dimethyl-labeling-based 4C quantitative PTM proteomics workflow in the study of dynamic acetylproteomic changes in Arabidopsis. This workflow integrates the dimethyl chemical labeling with chromatography-based acetylpeptide separation and enrichment followed by mass spectrometry (MS) analysis, the extracted ion chromatogram (XIC) quantitation-based computational analysis of mass spectrometry data to measure dynamic changes of acetylpeptide level using an in-house software program, named S<

SUBMITTER: Liu S 

PROVIDER: S-EPMC5930405 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature