Staining of E-selectin ligands on paraffin-embedded sections of tumor tissue.

ABSTRACT: BACKGROUND:The E-selectin ligands expressed by cancer cells mediate adhesion of circulating cancer cells to endothelial cells, as well as within tissue microenvironments important for tumor progression and metastasis. The identification of E-selectin ligands within cancer tissue could yield new biomarkers for patient stratification and aid in identifying novel therapeutic targets. The determinants of selectin ligands consist of sialylated tetrasaccharides, the sialyl Lewis X and A (sLeX and sLeA), displayed on protein or lipid scaffolds. Standardized procedures for immunohistochemistry make use of the antibodies against sLeX and/or sLeA. However, antibody binding does not define E-selectin binding activity. METHODS:In this study, we developed an immunohistochemical staining technique, usin

SUBMITTER: Carrascal MA 

PROVIDER: S-EPMC5930952 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature