Improved ethanol productivity from lignocellulosic hydrolysates by Escherichia coli with regulated glucose utilization.

ABSTRACT:

Background

Lignocellulosic ethanol could offer a sustainable source to meet the increasing worldwide demand for fuel. However, efficient and simultaneous metabolism of all types of sugars in lignocellulosic hydrolysates by ethanol-producing strains is still a challenge.

Results

An engineered strain Escherichia coli B0013-2021HPA with regulated glucose utilization, which could use all monosaccharides in lignocellulosic hydrolysates except glucose for cell growth and glucose for ethanol production, was constructed. In E. coli B0013-2021HPA, pta-ackA, ldhA and pflB were deleted to block the formation of acetate, lactate and formate and additional three mutations at glk, ptsG and manZ generated to block the glucose uptake and catabolism, followed by the replacement of the wild-t

SUBMITTER: Sun J 

PROVIDER: S-EPMC5930954 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature