Mapping of the three-dimensional lymphatic microvasculature in bladder tumours using light-sheet microscopy.

ABSTRACT:

Background

Cancers are heterogeneous and contain various types of irregular structures that can go undetected when examining them with standard two-dimensional microscopes. Studies of intricate networks of vasculature systems, e.g., the tumour lymphatic microvessels, benefit largely from three-dimensional imaging data analysis.

Methods

The new DIPCO (Diagnosing Immunolabeled Paraffin-Embedded Cleared Organs) imaging platform uses three-dimensional light-sheet microscopy and whole-mount immunolabelling of cleared samples to study proteins and micro-anatomies deep inside of tumours.

Results

Here, we uncovered the whole three-dimensional lymphatic microvasculature of formalin-fixed paraffin-embedded (FFPE) tumours from a cohort of 30 patients with bladder cancer. Our res

SUBMITTER: Tanaka N 

PROVIDER: S-EPMC5931093 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature