Metabolic systems analysis of LPS induced endothelial dysfunction applied to sepsis patient stratification.

ABSTRACT: Endothelial dysfunction contributes to sepsis outcome. Metabolic phenotypes associated with endothelial dysfunction are not well characterised in part due to difficulties in assessing endothelial metabolism in situ. Here, we describe the construction of iEC2812, a genome scale metabolic reconstruction of endothelial cells and its application to describe metabolic changes that occur following endothelial dysfunction. Metabolic gene expression analysis of three endothelial subtypes using iEC2812 suggested their similar metabolism in culture. To mimic endothelial dysfunction, an in vitro sepsis endothelial cell culture model was established and the metabotypes associated with increased endothelial permeability and glycocalyx loss after inflammatory stimuli were quantitatively defined through

SUBMITTER: McGarrity S 

PROVIDER: S-EPMC5931560 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature