Sacrificial-layer free transfer of mammalian cells using near infrared femtosecond laser pulses.

ABSTRACT: Laser-induced cell transfer has been developed in recent years for the flexible and gentle printing of cells. Because of the high transfer rates and the superior cell survival rates, this technique has great potential for tissue engineering applications. However, the fact that material from an inorganic sacrificial layer, which is required for laser energy absorption, is usually transferred to the printed target structure, constitutes a major drawback of laser based cell printing. Therefore alternative approaches using deep UV laser sources and protein based acceptor films for energy absorption, have been introduced. Nevertheless, deep UV radiation can introduce DNA double strand breaks, thereby imposing the risk of carcinogenesis. Here we present a method for the laser-induced transfer of

SUBMITTER: Zhang J 

PROVIDER: S-EPMC5931680 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature