Temporal Variability of Oral Microbiota over 10 Months and the Implications for Future Epidemiologic Studies.

ABSTRACT: Background: Few studies have prospectively evaluated the association between oral microbiota and health outcomes. Precise estimates of the intrasubject microbial metric stability will allow better study planning. Therefore, we conducted a study to evaluate the temporal variability of oral microbiota.Methods: Forty individuals provided six oral samples using the OMNIgene ORAL kit and Scope mouthwash oral rinses approximately every two months over 10 months. DNA was extracted using the QIAsymphony and the V4 region of the 16S rRNA gene was amplified and sequenced using the MiSeq. To estimate temporal variation, we calculated intraclass correlation coefficients (ICCs) for a variety of metrics and examined stability after clustering samples into distinct community types using Dir

SUBMITTER: Vogtmann E 

PROVIDER: S-EPMC5932224 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature