Investigation of Substrate Recognition and Biosynthesis in Class IV Lanthipeptide Systems.

ABSTRACT: Lanthipeptides belong to the family of ribosomally synthesized and post-translationally modified peptides (RiPPs) and are subdivided into four classes. The first two classes have been heavily studied, but less is known about classes III and IV. The lanthipeptide synthetases of classes III and IV share a similar organization of protein domains: A lyase domain at the N-terminus, a central kinase domain, and a C-terminal cyclase domain. Here, we provide deeper insight into class IV enzymes (LanLs). A series of putative producer strains was screened to identify production conditions of four new venezuelin-like lanthipeptides, and an Escherichia coli based heterologous production system was established for a fifth. The latter not only allowed production of fully modified core peptide but was al

SUBMITTER: Hegemann JD 

PROVIDER: S-EPMC5932250 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature