Dependence receptor UNC5A restricts luminal to basal breast cancer plasticity and metastasis.

ABSTRACT:

Background

The majority of estrogen receptor-positive (ERα⁺) breast cancers respond to endocrine therapies. However, resistance to endocrine therapies is common in 30% of cases, which may be due to altered ERα signaling and/or enhanced plasticity of cancer cells leading to breast cancer subtype conversion. The mechanisms leading to enhanced plasticity of ERα-positive cancer cells are unknown.

Methods

We used short hairpin (sh)RNA and/or the CRISPR/Cas9 system to knockdown the expression of the dependence receptor UNC5A in ERα⁺ MCF7 and T-47D cell lines. RNA-seq, quantitative reverse transcription polymerase chain reaction, chromatin immunoprecipitation, and Western blotting were used to measure the effect of UNC5A knockdown on basal and estradiol (E2)-r

SUBMITTER: Padua MB 

PROVIDER: S-EPMC5932758 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature