Nicotinamide Adenine Dinucleotide Phosphate Oxidase Expression Is Differentially Regulated to Favor a Pro-oxidant State That Contributes to Postoperative Adhesion Development.

ABSTRACT: We have previously reported that superoxide (O₂^•-) contributes to the development of postoperative adhesions. In this study, we determined whether O₂^•- generating nicotinamide adenine dinucleotide phosphate oxidase (NOX) is differentially expressed in normal peritoneal and adhesion fibroblasts and tissues. The NOX isoforms were measured utilizing Western blot, immunohistochemistry, high-performance liquid chromatography, and real-time reverse transcription polymerase chain reaction. Expression and activity of NOX were found to be significantly higher in adhesion tissues and cells than that in normal peritoneal tissues and cells (P < .05). Levels of NOX2, NOX4, NOX activating protein 1, DUOX1, p47^phox, and p22^phox messenger R

SUBMITTER: Fletcher NM 

PROVIDER: S-EPMC5933105 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature