ortho and para chromophores of green fluorescent protein: controlling electron emission and internal conversion.

ABSTRACT: Green fluorescent protein (GFP) continues to play an important role in the biological and biochemical sciences as an efficient fluorescent probe and is also known to undergo light-induced redox transformations. Here, we employ photoelectron spectroscopy and quantum chemistry calculations to investigate how the phenoxide moiety controls the competition between electron emission and internal conversion in the isolated GFP chromophore anion, following photoexcitation with ultraviolet light in the range 400-230 nm. We find that moving the phenoxide group from the para position to the ortho position enhances internal conversion back to the ground electronic state but that adding an additional OH group to the para chromophore, at the ortho position, impedes internal c

SUBMITTER: McLaughlin C 

PROVIDER: S-EPMC5933426 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature