Accurate label-free 3-part leukocyte recognition with single cell lens-free imaging flow cytometry.

ABSTRACT: Three-part white blood cell differentials which are key to routine blood workups are typically performed in centralized laboratories on conventional hematology analyzers operated by highly trained staff. With the trend of developing miniaturized blood analysis tool for point-of-need in order to accelerate turnaround times and move routine blood testing away from centralized facilities on the rise, our group has developed a highly miniaturized holographic imaging system for generating lens-free images of white blood cells in suspension. Analysis and classification of its output data, constitutes the final crucial step ensuring appropriate accuracy of the system. In this work, we implement reference holographic images of single white blood cells in suspension, in order to establish an accura

SUBMITTER: Li Y 

PROVIDER: S-EPMC5933530 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature