An optimized electroporation approach for efficient CRISPR/Cas9 genome editing in murine zygotes.

ABSTRACT: Electroporation of zygotes represents a rapid alternative to the elaborate pronuclear injection procedure for CRISPR/Cas9-mediated genome editing in mice. However, current protocols for electroporation either require the investment in specialized electroporators or corrosive pre-treatment of zygotes which compromises embryo viability. Here, we describe an easily adaptable approach for the introduction of specific mutations in C57BL/6 mice by electroporation of intact zygotes using a common electroporator with synthetic CRISPR/Cas9 components and minimal technical requirement. Direct comparison to conventional pronuclear injection demonstrates significantly reduced physical damage and thus improved embryo development with successful genome editing in up to 100% of living offspring. Hence, o

SUBMITTER: Troder SE 

PROVIDER: S-EPMC5933690 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature