Chromosomal barcoding as a tool for multiplexed phenotypic characterization of laboratory evolved lineages.

ABSTRACT: Adaptive laboratory evolution is an important tool to evolve organisms to increased tolerance towards different physical and chemical stress. It is applied to study the evolution of antibiotic resistance as well as genetic mechanisms underlying improvements in production strains. Adaptive evolution experiments can be automated in a high-throughput fashion. However, the characterization of the resulting lineages can become a time consuming task, when the performance of each lineage is evaluated individually. Here, we present a novel method for the markerless insertion of randomized genetic barcodes into the genome of Escherichia coli using a novel dual-auxotrophic selection approach. The barcoded E. coli library allows multiplexed phenotyping of evolved strains in pooled competition experim

SUBMITTER: Jahn LJ 

PROVIDER: S-EPMC5934437 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature