A gene expression profile analysis of the differentiation of muscle-derived stem cells into smooth muscle cells from sheep.

ABSTRACT: OBJECTIVE:To analyze gene expression profiles analysis during the differentiation of sheep muscle-derived stem cells (MDSCs) into smooth muscle cells (SMCs) in sheep. METHODS:A modified preplate technique was employed to isolation of the MDSCs from sheep. The MDSCs were subjected to flow cytometry analysis targeting CD44, CD31, CD45, CD14, and CD49f and were treated with TGF-β1 at a concentration of 10 ng/ml for ten days. The expression levels of smooth muscle α-actin (α-SMA) and calponin after treatment with TGF-β1 were determined by western blotting and immunofluorescence staining. A microarray analysis was performed to screen for differentially expressed genes (DEGs) during MDSC differentiation using total RNA extracted from MDSCs and SMCs generated from MDSCs. Molecule Annotation Syste

SUBMITTER: Tang X 

PROVIDER: S-EPMC5934578 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature