Pursuing origins of (poly)ethylene glycol-induced G-quadruplex structural modulations.

ABSTRACT: Molecular crowding conditions provided by high concentration of cosolutes are utilized for characterization of biomolecules in cell-mimicking environment and development of drug-delivery systems. In this context, (poly)ethylene glycols are often used for studying non-canonical DNA structures termed G-quadruplexes, which came into focus by emerging structural biology findings and new therapeutic drug design approaches. Recently, several reports were made arguing against using (poly)ethylene glycols in role of molecular crowding agents due to their direct impact on DNA G-quadruplex stability and topology. However, the available data on structural details underlying DNA interaction is very scarce and thus limits in-depth comprehension. Herein, structural and thermodynamic analyses were strate

SUBMITTER: Trajkovski M 

PROVIDER: S-EPMC5934638 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature