Targeting IS608 transposon integration to highly specific sequences by structure-based transposon engineering.

ABSTRACT: Transposable elements are efficient DNA carriers and thus important tools for transgenesis and insertional mutagenesis. However, their poor target sequence specificity constitutes an important limitation for site-directed applications. The insertion sequence IS608 from Helicobacter pylori recognizes a specific tetranucleotide sequence by base pairing, and its target choice can be re-programmed by changes in the transposon DNA. Here, we present the crystal structure of the IS608 target capture complex in an active conformation, providing a complete picture of the molecular interactions between transposon and target DNA prior to integration. Based on this, we engineered IS608 variants to direct their integration specifically to various 12/17-nt long target sites by extending the base pair in

SUBMITTER: Morero NR 

PROVIDER: S-EPMC5934647 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature