Pentacyclic adenine: a versatile and exceptionally bright fluorescent DNA base analogue.

ABSTRACT: Emissive base analogs are powerful tools for probing nucleic acids at the molecular level. Herein we describe the development and thorough characterization of pentacyclic adenine (pA), a versatile base analog with exceptional fluorescence properties. When incorporated into DNA, pA pairs selectively with thymine without perturbing the B-form structure and is among the brightest nucleobase analogs reported so far. Together with the recently established base analog acceptor qA_nitro, pA allows accurate distance and orientation determination via Förster resonance energy transfer (FRET) measurements. The high brightness at emission wavelengths above 400 nm also makes it suitable for fluorescence microscopy, as demonstrated by imaging of single liposomal constructs coated with c

SUBMITTER: Bood M 

PROVIDER: S-EPMC5934695 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature