RSM based optimization of nutritional conditions for cellulase mediated Saccharification by Bacillus cereus.

ABSTRACT:

Background

Cellulases are enzyme which have potential applications in various industries. Researchers are looking for potential cellulolytic bacterial strains for industrial exploitation. In this investigation, cellulase production of Bacillus cereus was explored while attacking poplar twigs. The bacterium was isolated from the gut of freshwater fish, Labeo rohita and identified by 16S rRNA gene sequencing technology. Various nutritional conditions were screened and optimized through response surface methodology. Initially, Plackett-Burman design was used for screening purpose and optimization was conducted through Box-Bhenken design.

Results

The maximum cellulase production occurred at 0.5% yeast extract, 0.09% MgSO₄, 0.04% peptone, 2% poplar waste

SUBMITTER: Tabssum F 

PROVIDER: S-EPMC5934882 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature