Quantification and localization of oncogenic receptor tyrosine kinase variant transcripts using molecular inversion probes.

ABSTRACT: Oncogenic membrane receptor tyrosine kinases such as MET and EGFR, or auto-active variants thereof, are important targets for cancer precision therapy. Targeted inhibition of these oncogenic receptors however invariably leads to resistance, resulting from acquisition of resistance-inducing mutations or from selective outgrowth of a priori resistant tumour cells. Most applied molecular protocols cannot distinguish between intracellular and intercellular heterogeneity of oncogene (variant) expression, which may lead to misinterpretation of the molecular make-up of a cancer and suboptimal application of targeted therapies. We here combined two related techniques to allow semiquantitative and localized in situ detection of specific transcript splice variants using single molecule molecular inv

SUBMITTER: van den Heuvel CNAM 

PROVIDER: S-EPMC5935718 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature