Metabolic engineering of Saccharomyces cerevisiae for efficient production of glucaric acid at high titer.

ABSTRACT:

Background

Glucaric acid is a high-value-added chemical that can be used in various fields. Because chemical oxidation of glucose to produce glucaric acid is not environmentally friendly, microbial production has attracted increasing interest recently. Biological pathways to synthesize glucaric acid from glucose in both Escherichia coli and Saccharomyces cerevisiae by co-expression of genes encoding myo-inositol-1-phosphate synthase (Ino1), myo-inositol oxygenase (MIOX), and uronate dehydrogenase (Udh) have been constructed. However, low activity and instability of MIOX from Mus musculus was proved to be the bottleneck in this pathway.

Results

A more stable miox4 from Arabidopsis thaliana was chosen in the present study. In addition, high copy delta-sequence integration of m

SUBMITTER: Chen N 

PROVIDER: S-EPMC5935971 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature