Evaluation of commercially available small RNASeq library preparation kits using low input RNA.

ABSTRACT:

Background

Evolving interest in comprehensively profiling the full range of small RNAs present in small tissue biopsies and in circulating biofluids, and how the profile differs with disease, has launched small RNA sequencing (RNASeq) into more frequent use. However, known biases associated with small RNASeq, compounded by low RNA inputs, have been both a significant concern and a hurdle to widespread adoption. As RNASeq is becoming a viable choice for the discovery of small RNAs in low input samples and more labs are employing it, there should be benchmark datasets to test and evaluate the performance of new sequencing protocols and operators. In a recent publication from the National Institute of Standards and Technology, Pine et al., 2018, the investigators used a commercially a

SUBMITTER: Yeri A 

PROVIDER: S-EPMC5936030 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature