Dissecting the Acid Stress Response of Rhizobium tropici CIAT 899.

ABSTRACT: Rhizobium tropici CIAT899 is a nodule-forming α-proteobacterium displaying intrinsic resistance to several abiotic stress conditions such as low pH and high temperatures, which are common in tropical environments. It is a good competitor for Phaseolus vulgaris (common bean) nodule occupancy at low pH values, however little is known about the genetic and physiological basis of the tolerance to acidic conditions. To identify genes in R. tropici involved in pH stress response we combined two different approaches: (1) A Tn5 mutant library of R. tropici CIAT899 was screened and 26 acid-sensitive mutants were identified. For 17 of these mutants, the transposon insertion sites could be identified. (2) We also studied the transcriptomes of cells grown under diffe

SUBMITTER: Guerrero-Castro J 

PROVIDER: S-EPMC5936775 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature