Metabolomic Fingerprints of Individual Algal Cells Using the Single-Probe Mass Spectrometry Technique.

ABSTRACT: Traditional approaches for the assessment of physiological responses of microbes in the environment rely on bulk filtration techniques that obscure differences among populations as well as among individual cells. Here, were report on the development on a novel micro-scale sampling device, referred to as the "Single-probe," which allows direct extraction of metabolites from living, individual phytoplankton cells for mass spectrometry (MS) analysis. The Single-probe is composed of dual-bore quartz tubing which is pulled using a laser pipette puller and fused to a silica capillary and a nano-ESI. For this study, we applied Single-probe MS technology to the marine dinoflagellate Scrippsiella trochoidea, assaying cells grown under different illumination levels and under nitrogen (N) limi

SUBMITTER: Sun M 

PROVIDER: S-EPMC5936784 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature