Ampicillin permeation across OmpF, the major outer-membrane channel in Escherichia coli.

ABSTRACT: The outer cell wall of the Gram-negative bacteria is a crucial barrier for antibiotics to reach their target. Here, we show that the chemical stability of the widely used antibiotic ampicillin is a major factor in the permeation across OmpF to reach the target in the periplasm. Using planar lipid bilayers we investigated the interactions and permeation of OmpF with ampicillin, its basic pH-induced primary degradation product (penicilloic acid), and the chemically more stable benzylpenicillin. We found that the solute-induced ion current fluctuation is 10 times higher with penicilloic acid than with ampicillin. Furthermore, we also found that ampicillin can easily permeate through OmpF, at an ampicillin gradient of 10 μm and a conductance of G_amp ≅ 3.8 fS, with a flux rate

SUBMITTER: Ghai I 

PROVIDER: S-EPMC5936826 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature