Profiling the Urinary Microbiome in Men with Positive versus Negative Biopsies for Prostate Cancer.

ABSTRACT:

Purpose

Studies demonstrating bacterial DNA and cultivable bacteria in urine samples have challenged the clinical dogma that urine is sterile. Furthermore, studies now indicate that dysbiosis of the urinary microbiome is associated with pathological conditions. We propose that the urinary microbiome may influence chronic inflammation observed in the prostate, leading to prostate cancer development and progression. Therefore, we profiled the urinary microbiome in men with positive vs negative biopsies for prostate cancer.

Materials and methods

Urine was collected from men prior to biopsy for prostate cancer. DNA was extracted from urine pellet samples and subjected to bacterial 16S rDNA Illumina® sequencing and 16S rDNA quantitative polymerase chain reaction. We determined th

SUBMITTER: Shrestha E 

PROVIDER: S-EPMC5937117 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature