Quantifying the Precision of Single-Molecule Torque and Twist Measurements Using Allan Variance.

ABSTRACT: Single-molecule manipulation techniques have provided unprecedented insights into the structure, function, interactions, and mechanical properties of biological macromolecules. Recently, the single-molecule toolbox has been expanded by techniques that enable measurements of rotation and torque, such as the optical torque wrench (OTW) and several different implementations of magnetic (torque) tweezers. Although systematic analyses of the position and force precision of single-molecule techniques have attracted considerable attention, their angle and torque precision have been treated in much less detail. Here, we propose Allan deviation as a tool to systematically quantitate angle and torque precision in single-molecule measurements. We apply the Allan variance method to experimental data f

SUBMITTER: van Oene MM 

PROVIDER: S-EPMC5937147 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature