Dynamics of a Protein Chain Motor Driving Helical Bacteria under Stress.

ABSTRACT: The wall-less, helical bacterial genus Spiroplasma has a unique propulsion system; it is not driven by propeller-like flagella but by a membrane-bound, cytoplasmic, linear motor that consists of a contractile chain of identical proteins spanning the entire cell length. By a coordinated spread of conformational changes of the proteins, kinks propagate in pairs along the cell body. However, the mechanisms for the initiation or delay of kinks and their coordinated spread remain unclear. Here, we show how we manipulate the initiation of kinks, their propagation velocities, and the time between two kinks for a single cell trapped in an optical line potential. By interferometric three-dimensional shape tracking, we measured the cells' deformations in response to various external stress situation

SUBMITTER: Roth J 

PROVIDER: S-EPMC5937200 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature