Extended-Depth 3D Super-Resolution Imaging Using Probe-Refresh STORM.

ABSTRACT: Single-molecule localization microscopy methods for super-resolution fluorescence microscopy such as STORM (stochastic optical reconstruction microscopy) are generally limited to thin three-dimensional (3D) sections (≤600 nm) because of photobleaching of molecules outside the focal plane. Although multiple focal planes may be imaged before photobleaching by focusing progressively deeper within the sample, image quality is compromised in this approach because the total number of measurable localizations is divided between detection planes. Here, we solve this problem on fixed samples by developing an imaging method that we call probe-refresh STORM (prSTORM), which allows bleached fluorophores to be straightforwardly replaced with nonbleached fluorophores. We accomplish this by immunostainin

SUBMITTER: Lin D 

PROVIDER: S-EPMC5937290 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature