Real-time Loop-mediated Isothermal Amplification (LAMP) of mgc2 Gene of Mycoplasma Gallisepticum.

ABSTRACT:

Introduction

Mycoplasma gallisepticum is considered the most pathogenic and economically significant avian Mycoplasma spp. for the worldwide poultry industry. The aim of this study was to develop a novel and sensitive real-time loop-mediated isothermal amplification (LAMP) assay based on the amplification of its mgc2 gene sequence for its rapid molecular detection in poultry.

Material and methods

Blood samples from 300 broiler and layer chickens were screened using a rapid serum agglutination (RSA) test. A real-time LAMP reaction was conducted with seropositive swab samples at 60ºC for 90 min in an ESEQuant tube scanner using 6-carboxyfluorescein as the reporting dye.

Results

The sensitivity of the developed assay was 10 fg/µL of DNA. The assay wa

SUBMITTER: Ehtisham-Ul-Haque S 

PROVIDER: S-EPMC5937342 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature