SGK1 Inhibits Autophagy in Murine Muscle Tissue.

ABSTRACT:

Background/aims

As autophagy is linked to several pathological conditions, like cancer and neurodegenerative diseases, it is crucial to understand its regulatory signaling network. In this study, we investigated the role of the serum- and glucocorticoid-induced protein kinase 1 (SGK1) in the control of autophagy.

Methods

To measure autophagic activity in vivo, we quantified the abundance of the autophagy conjugates LC3-PE (phosphatidylethanolamine) and ATG12-ATG5 in tissue extracts of SGK1 wild-type (Sgk1^+/+) and knockout (Sgk1^-/-) mice that were either fed or starved for 24 h prior sacrifice. In vitro, we targeted SGK1 by RNAi using GFP-WIPI1 expressing U-2 OS cells to quantify the numbers of cells displaying newly formed au

SUBMITTER: Zuleger T 

PROVIDER: S-EPMC5937381 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature