ATP depletion during mitotic arrest induces mitotic slippage and APC/C^Cdh1-dependent cyclin B1 degradation.

ABSTRACT: ATP depletion inhibits cell cycle progression, especially during the G1 phase and the G2 to M transition. However, the effect of ATP depletion on mitotic progression remains unclear. We observed that the reduction of ATP after prometaphase by simultaneous treatment with 2-deoxyglucose and NaN₃ did not arrest mitotic progression. Interestingly, ATP depletion during nocodazole-induced prometaphase arrest resulted in mitotic slippage, as indicated by a reduction in mitotic cells, APC/C-dependent degradation of cyclin B1, increased cell attachment, and increased nuclear membrane reassembly. Additionally, cells successfully progressed through the cell cycle after mitotic slippage, as indicated by EdU incorporation and time-lapse imaging. Although degradation of cyclin B during normal

SUBMITTER: Park YY 

PROVIDER: S-EPMC5938023 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature