Light-sheet microscopy with attenuation-compensated propagation-invariant beams.

ABSTRACT: Scattering and absorption limit the penetration of optical fields into tissue. We demonstrate a new approach for increased depth penetration in light-sheet microscopy: attenuation-compensation of the light field. This tailors an exponential intensity increase along the illuminating propagation-invariant field, enabling the redistribution of intensity strategically within a sample to maximize signal and minimize irradiation. A key attribute of this method is that only minimal knowledge of the specimen transmission properties is required. We numerically quantify the imaging capabilities of attenuation-compensated Airy and Bessel light sheets, showing that increased depth penetration is gained without compromising any other beam attributes. This powerful yet straightforward concept, combined

SUBMITTER: Nylk J 

PROVIDER: S-EPMC5938225 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature