Transcriptomic Profiling of Zebrafish Hair Cells Using RiboTag.

ABSTRACT: The zebrafish inner ear organs and lateral line neuromasts are comprised of a variety of cell types, including mechanosensitive hair cells. Zebrafish hair cells are evolutionarily homologous to mammalian hair cells, and have been particularly useful for studying normal hair cell development and function. However, the relative scarcity of hair cells within these complex organs, as well as the difficulty of fine dissection at early developmental time points, makes hair cell-specific gene expression profiling technically challenging. Cell sorting methods, as well as single-cell RNA-Seq, have proved to be very informative in studying hair cell-specific gene expression. However, these methods require that tissues are dissociated, the processing for which can lead to changes in gene expression p

SUBMITTER: Matern MS 

PROVIDER: S-EPMC5939014 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature