EIF1 Loop 2 interactions with Met-tRNA_i control the accuracy of start codon selection by the scanning preinitiation complex.

ABSTRACT: The eukaryotic 43S preinitiation complex (PIC), bearing initiator methionyl transfer RNA (Met-tRNA_i) in a ternary complex (TC) with eukaryotic initiation factor 2 (eIF2)-GTP, scans the mRNA leader for an AUG codon in favorable context. AUG recognition evokes rearrangement from an open PIC conformation with TC in a "P_OUT" state to a closed conformation with TC more tightly bound in a "P_IN" state. eIF1 binds to the 40S subunit and exerts a dual role of enhancing TC binding to the open PIC conformation while antagonizing the P_IN state, necessitating eIF1 dissociation for start codon selection. Structures of reconstituted PICs reveal juxtaposition of eIF1 Loop 2 with the Met-tRNA_i D loop in the P_IN state and predict a distortion

SUBMITTER: Thakur A 

PROVIDER: S-EPMC5939108 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature