Optimization of signal-to-noise ratio for efficient microarray probe design.

ABSTRACT:

Motivation

Target-specific hybridization depends on oligo-probe characteristics that improve hybridization specificity and minimize genome-wide cross-hybridization. Interplay between specific hybridization and genome-wide cross-hybridization has been insufficiently studied, despite its crucial role in efficient probe design and in data analysis.

Results

In this study, we defined hybridization specificity as a ratio between oligo target-specific hybridization and oligo genome-wide cross-hybridization. A microarray database, derived from the Genomic Comparison Hybridization (GCH) experiment and performed using the Affymetrix platform, contains two different types of probes. The first type of oligo-probes does not have a specific target on the genome and their hybridization sig

SUBMITTER: Matveeva OV 

PROVIDER: S-EPMC5939967 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature