Accurate estimation of isoelectric point of protein and peptide based on amino acid sequences.

ABSTRACT:

Motivation

In any macromolecular polyprotic system-for example protein, DNA or RNA-the isoelectric point-commonly referred to as the pI-can be defined as the point of singularity in a titration curve, corresponding to the solution pH value at which the net overall surface charge-and thus the electrophoretic mobility-of the ampholyte sums to zero. Different modern analytical biochemistry and proteomics methods depend on the isoelectric point as a principal feature for protein and peptide characterization. Protein separation by isoelectric point is a critical part of 2-D gel electrophoresis, a key precursor of proteomics, where discrete spots can be digested in-gel, and proteins subsequently identified by analytical mass spectrometry. Peptide fractionation according to their pI is al

SUBMITTER: Audain E 

PROVIDER: S-EPMC5939969 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature