Repeat-Specific Functions for the C-Terminal Domain of RNA Polymerase II in Budding Yeast.

ABSTRACT: The C-terminal domain (CTD) of the largest subunit of RNA polymerase II (RNAPII) is required to regulate transcription and to integrate it with other essential cellular processes. In the budding yeast Saccharomyces cerevisiae, the CTD of Rpb1p consists of 26 conserved heptad repeats that are post-translationally modified to orchestrate protein factor binding at different stages of the transcription cycle. A long-standing question in the study of the CTD is if there are any functional differences between the 26 repeats. In this study, we present evidence that repeats of identical sequence have different functions based on their position within the CTD. We assembled plasmids expressing Rpb1p with serine to alanine substitutions in three defined regions of the CTD and measured a range

SUBMITTER: Babokhov M 

PROVIDER: S-EPMC5940151 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature