Effects of Larval Density on Gene Regulation in <i>Caenorhabditis elegans</i> During Routine L1 Synchronization.

ABSTRACT: Bleaching gravid C. elegans followed by a short period of starvation of the L1 larvae is a routine method performed by worm researchers for generating synchronous populations for experiments. During the process of investigating dietary effects on gene regulation in L1 stage worms by single-worm RNA-Seq, we found that the density of resuspended L1 larvae affects expression of many mRNAs. Specifically, a number of genes related to metabolism and signaling are highly expressed in worms arrested at low density, but are repressed at higher arrest densities. We generated a GFP reporter strain based on one of the most density-dependent genes in our dataset - lips-15 - and confirmed that this reporter was expressed specifically in worms arrested at relatively low density. Finally, we

SUBMITTER: Chan IL 

PROVIDER: S-EPMC5940168 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature