BaiCD gene cluster abundance is negatively correlated with Clostridium difficile infection.

ABSTRACT:

Background

Clostridium difficile infection (CDI) is a major cause of hospital-acquired diarrhea. Secondary bile acids were shown to confer resistance to colonization by C. difficile. 7α-dehydroxylation is a key step in transformation of primary to secondary bile acids and required genes have been located in a single bile acid-inducible (bai) operon in C. scindens as well as in C. hiranonis, two Clostridium sp. recently reported to protect against C. difficile colonization.

Aim

To analyze baiCD gene abundance in C. difficile positive and negative fecal samples.

Material & methods

A species-specific qPCR for detecting baiCD genes was established. Fecal samples of patients with CDI, asymptomatic toxigenic C. difficile colonization (TCD), non-toxigenic C. difficile coloni

SUBMITTER: Solbach P 

PROVIDER: S-EPMC5940204 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature